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Obtention de souches conchylicoles performantes par polyploïdisation (2ème partie) ArchiMer
Gerard, Andre.
Following a phase of experimental system development, acquisition and improvement of triploidisation techniques carried out in 1989, the 1990 program divides into four main actions:1) significant production of diploid and triploid populations of cupped oysters and Pacific clams in order to compare their performances in French coastal waters2) first trials of triploidy induction in European clam3) development of a ploidy control technique at any stage of the animal life, through digital imaging4) summary of the first trials of tetraploidy induction in C. gigas and R. philippinarum
Tipo: Text Palavras-chave: Imagerie numérique; Ruditapes decussatus; Ruditapes philippinarum; Crassostrea gigas; Cytogénétique; Tétraploïdie; Triploïdie; Polyploïdie.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/rapport-3855.PDF
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Polyploidy in the Manila clam Ruditapes philippinarum. II. Chemical induction of tetraploid embryos ArchiMer
Diter, Alain; Dufy, Christian.
Tetraploidy was induced in embryos of Ruditapes philippinarum by treating the eggs with cytochalasin B. (CB). Two treatment times were found to be effective in inducing tetraplidy : 0 to 10 minutes after insemination (before formation of the first polar body) and 45 minutes after insemination (just before the first cleavage). Treatments, started 5 and 45 minutes after insemination, produced 64.4 ± 7.8% and 28.3 ± 2.3% tetraploid embrios, respectively, after 6 hours (on the average over 3 experiments). However, no tetraploids were detected in 4-month-old spat. Sperm entry delayed by the CB could be involved in induction of tetraploidy by early treatment.
Tipo: Text Palavras-chave: Ruditapes philippinarum; Bivalve; Tetraploidy; Cytochalasin B.; Ruditapes philippinarum; Bivalve; Tétraploïdie; Cytochalasine B.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/publication-2753.pdf
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